PRP Kits - Glanlab Instrument

Recommended Matching Centrifuges

Following centrifuge matching our PRP kits

TD5/TDL5 5000rpm, 4*250ml, Pre-cooling Design, Bench Top Universal Centrifuge, Smaller Size, Support Swing/Microplate/Fixed Rotor

Attributes

Max. Speed: 5000rpm
Max.Capacity: 4×250ml

Refrigerated Available: TDL5-Yes，TD5-NO

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Other recommended PRP centrifuges

YT4A Portable Low Speed Universal Centrifuge Clinical Centrifuge 4000rpm

Max. Speed: 4000rpm

Max.Capacity: 6×50ml

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YT5A 5000rpm Series Universal Hospital Centrifuge Blood Station

Max. Speed: 5000rpm

Max.Capacity: 4×300ml

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YT4C Portable Centrifuge Low Speed Favorite Price PRP Centrifuge

Max. Speed: 4000rpm

Max.Capacity: 8x20ml

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YXPRP PRP Centrifuge Stem Cells Centrifuge

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YT6 Table Top Centrifuge Widely Used in Laboratory,Clinic with CE/ISO

Max. Speed: 6000rpm

Max.Capacity: 4x180ml

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What’s the PRP ？

PRP , the full name is Platelet-Rich Plasma

Platelet rich Plasma is defined as a platelet concentration higher than the physiologic platelet concentration found in healthy whole blood

- Average normal platelet count =200,000 per micro liter
- Normal range =150,000 to 350,000 per micro liter

- PRP count=1,000,000 per micro liter(5X concentration)

The first step of the healing process is clot formation and platelet activation.After platelet activation, wound healing involves an intricate process that is often categorized into three overlapping phases: inflammation, proliferation, and remodeling.

How to use PRP kits？

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In a 30 cc syringe take 3 cc of anticoagulant, then draw 27cc of blood from a subject (final volume : 30 cc)
Inject the drawn blood to HPRP kit through the upper injection port until the blood level reaches the 30cc scale marked on the kit. (For easier injection, lean the kit)
Separate the plasma layer and red blood cell (RBC) layer by the first separation. (Prior to separation, balance the weight of kit with counterbalance) Generally keep 3000rpm for 3mins
*For subjects taking aspirin, antihypertensive drugs, with cardiac disease, anemia, or during menstruation, the separation should be done for 4 min at 1 ,000 RCF
After the first separation, identify the separation position of the plasma and the RBC layer. Then adjust the height of the separated boundary to the indicated point by pushing up or pulling down the adjusting knob located at the lower part of the Kit. (If the volume of the plasma layer or the separation is not quite well done, additional separation may be done for about 1-2 min at same RPM)
In order to block the plasma and the RBC layer completely, fasten the adjusting knob (clockwise) located at the lower part of the Kit. Then fasten the valve(clockwise) just above the adjusting knob. Finally fasten the adjusting knob(clockwise) again.
Put the fastened PRP Kit to the centrifuge with counterbalance for the second separation to enrich the concentrated platelet.
* Condition for the second separation 2 min at 3000 RPM at soft brake system
Place the PRP Kit in its upright position with care in order to prevent mixing of the separated PRP and PPP. Open the upper silicone lid on the Kit. Using a 10cc syringe with a long needle (> 5cm) take out the PPP layer slowly from the upper part leaving 5cc -8cc at the base.
Shake and mix the remaining 5 cc-8cc of the PRP layer.

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